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"Comprehensive Capture of FFPE RNA for RNA-Seq Using a Continuous Synthesis Chemistry and Post-Library Ribodepletion"

Gene expression of the transcriptome using common oncology biospecimens such as degraded FFPE is a substantial challenge to RNA-Seq adoption, which results in the loss of small and rare transcripts in transcriptome analysis.

A novel and streamlined workflow which combines library preparation and post-library ribosomal RNA (rRNA) depletion enables the ability to construct a more complex library from FFPE RNA that includes both long and short RNA biotypes and is therefore more representative of the complete transcriptome.

Download this white paper from Bio-Rad to learn about:

• How a novel enzyme leverages a retroviral reverse transcriptase and ligases in a single synthesis step
• Comprehensive capture of more RNA subtypes including the small RNA transcripts from FFPE samples
• Post-library ribodepletion minimizes the loss of the small RNAs

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